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8 of Clubs – Work/business problems that may have to do with jealousy. If the question concerns a particular romantic interest, this is considered a warning card – infidelity or separation is quite likely. Sometimes indicates that a third person is breaking into a relationship somehow. Sometimes indicates a negative or depressed person. 8 of Spades – Temptation, misfortune, danger, upsets. King of Spades – Dark-haired man; or a man with Air predominating in his chart. Diamonds Pentacles – Earth – Merchants Ace of Diamonds – Change; a message, often about money, and usually good news. 4 of Diamonds – Financial upswing; an older person may give good advice. If there are two Jokers with your deck of cards, and you choose to use the Joker in your readings, you need to add only one.

This is generally thought to be quite unfavourable. A trip taken now may result in a new friend or love interest. 2 of Diamonds – A business partnership; a change in relationship; gossip. Playing Card Combinations One significant difference between reading the Tarot and reading playing cards is the strong emphasis put on card combinations when reading playing cards.

8) Soy feliz de compartir cada instante de mi vida a tu lado. 12) Tarda una hora en conocerte y solo un dia en enamorarme. – It took me an hour to know you and only a day to fall in love.

– Your love inspires me, your tenderness touches me and your kisses drive me crazy.

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The fact is, trustworthy computer re-annotation remains far off on the horizon, so realistically we seek second generational tools that of hand annotation and allow rapid expert curation, as well as easy updating.

Gen Bank entries themselves are annotated very unevenly, depending on the knowledge and interest level of the sequencing lab (and no one is allowed to fix a bad annotation! Gen Bank is not curated: entries are only provide suggestions for genomic features such as promoters, alternative splicing of m RNAs, retrotransposons, pseudogenes, tandem duplications, synteny, and homology.

As time goes by, most annotations need updating to reflect the new information gained through rapid accumulation of genomic sequences.

Utilities : Align 1, 2 : Gene Bander : Tutorial Medline : OMIM : Mouse : Zfish : Fly : Worm : PNAS : Science : Nature : Genome Res : Mol Bio Evol : More Links Introduction to genome annotation 30 kb example: a dynein pseudogene Characterizing features Pseudogene annotation: the issues Assembling unfinished contigs How Gene Bander tracks are made How they did it in the old days Status and limitations of current tools Glossary, notation, and acronyms Published examples of good annotations Searching for disease genes: Hallervorden-Spatz Dynein pseudogene consisting solely of 3' UTR KIAA1628: annotation of unfinished contigs Tandem duplicate with chimeric intergenic exons Dating multiply nested retrotransposons Rps4X: inter-chromosomal superfamily Syntenic annotation across 5 species Older pseudogenes: ps IPP Comprehensive single gene annotation L7a: processed pseudogene with retained intron Gene-within-a-gene Gen Map00 integrated map A tough little gene Microsatellites in proteins 1, 2, 3 Predicting Cp G mutations1, 2, 3 Annotating 3'UTR across species Remote orthoologues by synteny Human disease genes: mapped to protein Human disease genes: partly mapped The human genome browser at UCSC Curated single gene mutation database NCBI/Gen Bank nightmare This tutorial follows an experienced annotator through real-life annotation of uncharacterized consequtive segments of the human genome -- look elsewhere for a dessicated classroom drill.

It is not intended as an introduction to molecular biology or basic Blast searching.


  1. AUS-e-TUTE is a science education website providing notes, quizzes, tests, exams, games, drills, worksheets, and syllabus study guides for high school science.

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